Kearney K R, Moore P B
J Mol Biol. 1983 Oct 25;170(2):381-402. doi: 10.1016/s0022-2836(83)80154-5.
That ribosomal subunits can exist in active and inactive functional states, and that subunits in the two states are interconvertible, has been known for some time (see Zamir et al., 1974). The magnitude of the conformational perturbation accompanying this functional transformation, however, was not known. In the present study 30 S and 50 S subunits in the two functional states have been compared by small-angle X-ray scattering. The results indicate that the structural differences between active and inactive subunits are small, at the limit of resolution of this technique. Model studies show that the data imply conformational differences at or below the limit of resolution of other physical methods now in use to examine the detailed structure of these particles.
核糖体亚基能够以活性和非活性功能状态存在，并且这两种状态的亚基可以相互转换，这一现象已为人所知有一段时间了（见Zamir等人，1974年）。然而，伴随这种功能转变的构象扰动程度尚不清楚。在本研究中，通过小角X射线散射对处于两种功能状态的30 S和50 S亚基进行了比较。结果表明，活性亚基和非活性亚基之间的结构差异很小，处于该技术的分辨率极限。模型研究表明，这些数据意味着在目前用于检查这些颗粒详细结构的其他物理方法的分辨率极限或以下存在构象差异。
