Mary J Y, Guiguet M, Sainteny F, Dumenil D, Frindel E
Exp Hematol. 1983 Apr;11(4):284-97.
The aim of this work was to analyze quantitatively experimental data, which suggest that CFUS determination can be manipulated by external fibers. This hypothesis was based on the comparison of the ratio of erythroid to granulocytic spleen colonies generated by normal bone marrow exposed to factors released by either untreated or arabinoside cytosine treated mouse bone marrow. To investigate mechanisms able to produce this modification of spleen colony histology, statistical analysis was performed by testing three different schemes of evolution of the number of splenic colonies according to this histology from normal to treated groups. The total number of colonies per spleen is similar in both groups, but a significant increase of the number of erythroid colonies per spleen and a significant decrease of the number of granulocytic colonies are observed in the treated group as compared to control. Bias due to the used experimental technique are investigated but could not explain the observed differences. A unique mechanism acting on only one committed stem cell population does not fit the experimental data. Although other possible mechanisms are suggested, the experimental observations can be interpreted as the consequence of a shift of CFUS differentiation toward the erythroid pathway at the expense of at least the granulocytic lineage due to some humoral factors, secreted by treated mouse bone marrow.
这项工作的目的是对实验数据进行定量分析，这些数据表明，CFUS的测定可受外部纤维的操控。该假设基于对正常骨髓暴露于未经处理或经阿糖胞苷处理的小鼠骨髓释放的因子后所产生的红系与粒系脾集落比率的比较。为了研究能够产生脾集落组织学这种改变的机制，通过测试根据这种组织学从正常组到处理组脾集落数量的三种不同演变方案进行了统计分析。两组中每个脾脏的集落总数相似，但与对照组相比，处理组中每个脾脏的红系集落数量显著增加，粒系集落数量显著减少。对所用实验技术造成的偏差进行了研究，但无法解释所观察到的差异。仅作用于一个定向干细胞群体的单一机制并不符合实验数据。尽管提出了其他可能的机制，但实验观察结果可解释为由于处理过的小鼠骨髓分泌的某些体液因子，CFUS分化向红系途径转变，至少以粒系谱系为代价。
