从产肠毒素大肠杆菌菌株18D中分离出的一种热稳定肠毒素的氨基酸序列。
Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigenic Escherichia coli strain 18D.
作者信息
Takao T, Hitouji T, Aimoto S, Shimonishi Y, Hara S, Takeda T, Takeda Y, Miwatani T
出版信息
FEBS Lett. 1983 Feb 7;152(1):1-5. doi: 10.1016/0014-5793(83)80469-4.
PMID:6341083
Abstract
A heat-stable enterotoxin produced by a strain of enterotoxigenic Escherichia coli 18D was purified by ion-exchange and reversed-phase high-pressure liquid chromatography. The amino acid sequence of the purified toxin was determined by Edman-degradation and a combination of fast atom bombardment mass spectrometry and carboxypeptidase digestion to be Asn-Thr-Phe-Tyr-Cys-Cys-Glu-Leu-Cys-Cys-Asn-Pro-Ala-Cys-Ala-Gly-Cys-Tyr.
摘要
一株产肠毒素大肠杆菌18D产生的一种热稳定肠毒素，通过离子交换和反相高压液相色谱法进行了纯化。采用埃德曼降解法、快原子轰击质谱法和羧肽酶消化相结合的方法，确定了纯化毒素的氨基酸序列为天冬酰胺-苏氨酸-苯丙氨酸-酪氨酸-半胱氨酸-半胱氨酸-谷氨酸-亮氨酸-半胱氨酸-半胱氨酸-天冬酰胺-脯氨酸-丙氨酸-半胱氨酸-丙氨酸-甘氨酸-半胱氨酸-酪氨酸。
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