大肠杆菌热稳定肠毒素编码基因的分离与核苷酸序列测定
Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli.
作者信息
Moseley S L, Hardy J W, Hug M I, Echeverria P, Falkow S
出版信息
Infect Immun. 1983 Mar;39(3):1167-74. doi: 10.1128/iai.39.3.1167-1174.1983.
Abstract
A gene encoding a heat-stable enterotoxin (ST) from an Escherichia coli strain isolated from a human with diarrhea was cloned and characterized by nucleotide sequence analysis. The gene was found to be partially homologous to a previously characterized ST gene from an E. coli strain of bovine origin. Hybridization studies showed that most ST-producing strains of E. coli isolated from humans with diarrhea possess genes highly homologous to either the ST gene from the bovine strain or the ST gene characterized in the present study.
摘要
从一名腹泻患者分离出的大肠杆菌菌株中，编码热稳定肠毒素（ST）的基因被克隆，并通过核苷酸序列分析进行了表征。发现该基因与先前表征的源自牛的大肠杆菌菌株的ST基因部分同源。杂交研究表明，从腹泻患者分离出的大多数产ST的大肠杆菌菌株，其基因与牛源菌株的ST基因或本研究中表征的ST基因高度同源。
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