产肠毒素大肠杆菌热稳定毒素II基因的特性分析及初步分子流行病学研究
Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers.
作者信息
Lee C H, Moseley S L, Moon H W, Whipp S C, Gyles C L, So M
出版信息
Infect Immun. 1983 Oct;42(1):264-8. doi: 10.1128/iai.42.1.264-268.1983.
Abstract
The gene encoding heat-stable toxin II (STII) was cloned into an Escherichia coli K-12 strain, and its nucleotide sequence was determined. The deduced amino acid sequence indicates that STII is synthesized within the cell as a 71-amino-acid protein and that neither the DNA nor amino acid sequence bears any similarity to that of heat-stable toxin I. A DNA fragment containing the STII gene was used to probe enterotoxigenic E. coli clinical isolates with various toxin phenotypes and was shown to be useful in detecting all STII and only STII producers.
摘要
编码热稳定毒素II（STII）的基因被克隆到大肠杆菌K-12菌株中，并测定了其核苷酸序列。推导的氨基酸序列表明，STII在细胞内作为一种71个氨基酸的蛋白质合成，其DNA和氨基酸序列与热稳定毒素I均无任何相似性。一个含有STII基因的DNA片段被用于检测具有各种毒素表型的产肠毒素大肠杆菌临床分离株，结果表明它可用于检测所有产生STII的菌株，且仅能检测出产生STII的菌株。
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Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers.产肠毒素大肠杆菌热稳定毒素II基因的特性分析及初步分子流行病学研究
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