Talle M A, Rao P E, Westberg E, Allegar N, Makowski M, Mittler R S, Goldstein G
Cell Immunol. 1983 May;78(1):83-99. doi: 10.1016/0008-8749(83)90262-9.
A series of seven monoclonal antibodies directed at determinants on human peripheral blood monocytes were produced and characterized. The antibodies were separated into three groups based on cell distribution and percentages of monocytes bearing antigen. Hybridoma antibodies, termed OKM1, OKM9, and OKM10, recognized antigen(s) expressed on the majority of adherent monocytes, null cells, and granulocytes. The second group, comprising OKM5 and OKM8, reacted with most adherent monocytes and platelets. OKM3 and OKM6, comprising a third group of antibodies, reacted with a subpopulation of adherent monocytes and platelets. OKM antibodies were not expressed on lymphocytes, thymocytes, and lymphoblastoid cells, with the exception of OKM3 which reacted with three B-cell lines. SDS gels of immunoprecipitates formed with OKM antibodies yielded the following tentative molecular weight results: OKM1 and OKM9 antigens appeared to be 160,000 (nonreduced) and 170,000 (reduced); OKM10 precipitated two polypeptides of 170,000 and 115,000 (reduced); OKM5 and OKM8 precipitated a single polypeptide of 88,000 (reduced, nonreduced); OKM6 antigen appeared to be 116,000 (nonreduced) and 130,000 (reduced).
制备并鉴定了一系列针对人外周血单核细胞决定簇的七种单克隆抗体。根据细胞分布和携带抗原的单核细胞百分比，将这些抗体分为三组。杂交瘤抗体OKM1、OKM9和OKM10识别大多数贴壁单核细胞、空细胞和粒细胞上表达的抗原。第二组包括OKM5和OKM8，与大多数贴壁单核细胞和血小板发生反应。OKM3和OKM6组成第三组抗体，与贴壁单核细胞和血小板的一个亚群发生反应。OKM抗体在淋巴细胞、胸腺细胞和淋巴母细胞样细胞上不表达，但OKM3与三种B细胞系发生反应。用OKM抗体形成的免疫沉淀物的SDS凝胶产生了以下初步分子量结果：OKM1和OKM9抗原在非还原状态下似乎为160,000，在还原状态下为170,000；OKM10沉淀出两条分子量分别为170,000和115,000（还原状态）的多肽；OKM5和OKM8沉淀出一条分子量为88,000的单一多肽（还原和非还原状态）；OKM6抗原在非还原状态下似乎为116,000，在还原状态下为130,000。
