Andersson A, Sandler S
Cryobiology. 1983 Apr;20(2):161-8. doi: 10.1016/0011-2240(83)90005-6.
Collagenase-isolated islets, which had been cultured for 1 week, were frozen at two different cooling rates. Islets frozen at 5 degrees C/min behaved from a functional point of view very similarly to that of nonfrozen, cultured control islets, except for a reduced maximal insulin secretory capacity and a reduced insulin content. Slowly frozen islets (0.5 degrees C/min), however, displayed reduced rates of both proinsulin biosynthesis and glucose oxidation. It is concluded that isolated islets can be cryopreserved with great success and that the methods of choice for viability tests are those characterizing the dynamics of insulin secretory capacity of the cryopreserved islets.
经胶原酶分离并培养1周的胰岛，以两种不同的降温速率冷冻。以5℃/分钟冷冻的胰岛，从功能角度来看，与未冷冻的培养对照胰岛非常相似，只是最大胰岛素分泌能力降低且胰岛素含量减少。然而，缓慢冷冻的胰岛（0.5℃/分钟）显示胰岛素原生物合成和葡萄糖氧化速率均降低。结论是分离的胰岛可以成功地进行冷冻保存，并且活力测试的首选方法是那些表征冷冻保存胰岛胰岛素分泌能力动态的方法。
