Stiller R L, Bennett J E, Scholer H J, Wall M, Polak A, Stevens D A
J Infect Dis. 1983 Jun;147(6):1070-7. doi: 10.1093/infdis/147.6.1070.
The in vitro susceptibility of Candida albicans isolates to flucytosine was compared to therapeutic effect in experimental murine candidiasis (candidosis). Four groups of 10 isolates were chosen, based upon their broth dilution minimal inhibitory concentrations (MICs), from a group of 402 isolates from patients without prior flucytosine therapy. Group I MICs were less than 12.5 micrograms/ml after seven days, whereas group II, III, and IV MICs exceeded 12.5 micrograms/ml on days 7, 2, and 1, respectively. Pilot experiments selected challenge inocula of similar virulence. Mice were infected intravenously and given various flucytosine doses. Significant prolongation of survival correlated with MICs and with agar disk-diffusion zone diameters (P less than 0.05). In vivo response to therapy was more favorable for group I isolates compared with group IV isolates (P less than 0.01). The present study demonstrates in this animal model that in vitro susceptibility does correlate with in vivo response to therapy, although exceptions occur with individual isolates.
将白色念珠菌分离株对氟胞嘧啶的体外敏感性与实验性小鼠念珠菌病（念珠菌感染）的治疗效果进行了比较。根据其肉汤稀释最低抑菌浓度（MIC），从402例未接受过氟胞嘧啶治疗的患者分离株中选取了四组，每组10株。第一组在7天后MIC小于12.5微克/毫升，而第二组、第三组和第四组的MIC分别在第7天、第2天和第1天超过12.5微克/毫升。预实验选择了毒力相似的攻击接种物。小鼠经静脉感染并给予不同剂量的氟胞嘧啶。生存时间的显著延长与MIC以及琼脂纸片扩散区直径相关（P<0.05）。与第四组分离株相比，第一组分离株对治疗的体内反应更有利（P<0.01）。本研究在该动物模型中表明，体外敏感性确实与体内治疗反应相关，尽管个别分离株存在例外情况。
