杆状病毒复制：[E]-5-(2-溴乙烯基)-2'-脱氧尿苷对粉纹夜蛾多核型多角体病毒的抑制作用
Baculovirus replication: inhibition of Trichoplusia ni multiple nuclear polyhedrosis virus by [E]-5-(2-bromovinyl)-2'-deoxyuridine.
作者信息
Wang X, Lescott T, De Clercq E, Kelly D C
出版信息
J Gen Virol. 1983 Jun;64 (Pt 6):1221-7. doi: 10.1099/0022-1317-64-6-1221.
[E]-5-(2-bromovinyl)-2'-deoxyuridine (BVdU) inhibits the replication of the baculovirus Trichoplusia ni multiple nucleocapsid nuclear polyhedrosis virus in Spodoptera frugiperda cells. Virus-specific DNA synthesis and late protein synthesis are suppressed by the drug. BVdU is phosphorylated by deoxythymidine (deoxycytidine) kinase present in both uninfected and virus-infected cells, and in its 5'-triphosphate form it inhibits DNA polymerase activity in virus-infected cells. The effect of the BVdU is not completely reversible. Phosphonoacetic acid, phosphonoformic acid and Acyclovir have no effect on baculovirus replication. Acyclovir fails to compete with deoxycytidine and thymidine as substrates for pyrimidine deoxynucleoside kinase in virus-infected and uninfected cells.
[E]-5-(2-溴乙烯基)-2'-脱氧尿苷（BVdU）可抑制杆状病毒草地贪夜蛾多核衣壳核型多角体病毒在草地贪夜蛾细胞中的复制。该药物可抑制病毒特异性DNA合成和晚期蛋白质合成。BVdU可被未感染和病毒感染细胞中存在的脱氧胸苷（脱氧胞苷）激酶磷酸化，其5'-三磷酸形式可抑制病毒感染细胞中的DNA聚合酶活性。BVdU的作用并非完全可逆。膦乙酸、膦甲酸和阿昔洛韦对杆状病毒复制无影响。在病毒感染和未感染的细胞中，阿昔洛韦无法与脱氧胞苷和胸苷竞争作为嘧啶脱氧核苷激酶的底物。
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