Phoenix P, Melançon P, Brakier-Gingras L
Mol Gen Genet. 1983;189(1):123-8. doi: 10.1007/BF00326064.
Neomycin-resistant mutants of Escherichia coli K12 were selected on a minimal succinate medium containing neomycin. In an in vitro poly(U)-dependent protein synthesizing assay, the ribosomes from the mutant strains showed lower levels of misreading than wild-type ribosomes in the presence of neomycin or other error-promoting aminoglycoside antibiotics or ethanol. The mutation was shown to affect a component of the 30S subunit and to facilitate the dissociation of ribosomes into subunits. It is suggested that an impairment in the subunit interaction may be responsible for the observed restriction of the stimulation of misreading.
在含有新霉素的基本琥珀酸盐培养基上筛选出大肠杆菌K12的耐新霉素突变体。在体外聚（U）依赖性蛋白质合成试验中，在新霉素、其他促进错误的氨基糖苷类抗生素或乙醇存在的情况下，突变菌株的核糖体比野生型核糖体表现出更低水平的错读。已证明该突变影响30S亚基的一个组分，并促进核糖体解离为亚基。有人提出，亚基相互作用的损害可能是观察到的错读刺激受限的原因。
