Nicoletti M, Bertani G
Mol Gen Genet. 1983;189(2):343-7. doi: 10.1007/BF00337829.
The chromosome of the temperate bacteriophage P2 and that of the plasmid pBR322 have been joined in vitro after treatment with restriction endonuclease EcoRI. The fusion product - a phasmid - can behave as a plasmid, as a phage and as a prophage. It can replicate its DNA under the control of either the specific replication mechanism of the parent phage in a polA mutant or that of the parent plasmid in a rep mutant. Several interesting interactions between the two replication modes are indicated. In particular, phage particles may be produced even when the phage mode of DNA replication is blocked, and this throws new light on the involvement of the early gene A in the regulation of late gene expression in phage P2.
在用限制性内切酶EcoRI处理后，温和噬菌体P2的染色体与质粒pBR322的染色体已在体外连接。融合产物——一种噬菌粒——可以表现为质粒、噬菌体和原噬菌体。它可以在polA突变体中受亲本噬菌体的特定复制机制控制，或在rep突变体中受亲本质粒的特定复制机制控制来复制其DNA。这表明了两种复制模式之间的几种有趣的相互作用。特别是，即使DNA复制的噬菌体模式被阻断，也可能产生噬菌体颗粒，这为早期基因A参与噬菌体P2晚期基因表达的调控提供了新的线索。
