Mordelet-Dambrine M, Marrache F, Leynadier F, Durieux P, Huchon G, Chretien J
Pathol Biol (Paris). 1983 Jan;31(1):49-51.
The human basophil degranulation test (TDBH) is performed using a concentrated cell suspension enriched for polymorphonuclear basophils. This preparation is placed in wells on a glass slide with an allergen solution of decreasing concentration. After incubation the cells are fixed in the wells and stained with toluidine blue. Preparations containing fewer metachromatic basophils than in control preparation of the same cell suspension without antigen contain degranulated basophils and in this way a degranulation index can be calculated. By considering the variability in the number of basophils between duplicate control wells from the same cell suspension, it can be shown that the test can be considered to be positive only if the number of degranulated basophils equals or exceeds 50% of the total number of basophils. This variance can be attributed to the subjectivity of the reader, and to differences in the differential cell counts from well to well. On the other hand, the presence of atopic allergy had no effect on the basophil counts.
人类嗜碱性粒细胞脱颗粒试验（TDBH）是使用富含多形核嗜碱性粒细胞的浓缩细胞悬液进行的。将这种制剂置于载玻片的孔中，并加入浓度递减的变应原溶液。孵育后，将细胞固定在孔中，并用甲苯胺蓝染色。与不含抗原的相同细胞悬液的对照制剂相比，含有较少异染性嗜碱性粒细胞的制剂含有脱颗粒的嗜碱性粒细胞，通过这种方式可以计算脱颗粒指数。考虑到来自同一细胞悬液的重复对照孔之间嗜碱性粒细胞数量的变异性，可以表明只有当脱颗粒嗜碱性粒细胞的数量等于或超过嗜碱性粒细胞总数的50%时，该试验才能被认为是阳性的。这种差异可归因于读数者的主观性，以及各孔之间细胞分类计数的差异。另一方面，特应性过敏的存在对嗜碱性粒细胞计数没有影响。
