Budka H, Shah K V
Prog Clin Biol Res. 1983;105:299-309.
Brain tissue of 36 patients with definite PML (showing histologically the pathognomonic "PML cells") and of 12 cases with possible PML (showing patchy demyelination without PML cells) were studied for the presence of papovavirus antigens. A cross-reactive genus-specific serum and a serum raised against JCV were used in the peroxidase-antiperoxidase technique on formalin-fixed paraffin sections of autopsy brain. There was some variability in intensity of staining between the cases and between the sera. Papovavirus antigen-containing cells--mainly PML cells--were found in 35 definite and in one possible PML case. Twenty-nine of these brains reacted with anti-JCV serum. In five brains, antigen deposits were prominent in cytoplasm and processes of astrocytes in some areas of PML lesions. Giant astrocytes demonstrated viral antigens in six cases, indicating that these cells are indeed infected in PML. There was no staining of nerve cells or endothelial cells.
对36例确诊为进行性多灶性白质脑病（组织学显示具有特征性的“PML细胞”）和12例可能为进行性多灶性白质脑病（显示斑片状脱髓鞘但无PML细胞）患者的脑组织进行了乳头多瘤空泡病毒抗原检测。采用交叉反应性属特异性血清和抗JCV血清，运用过氧化物酶-抗过氧化物酶技术，对尸检脑的福尔马林固定石蜡切片进行检测。病例之间以及血清之间的染色强度存在一定差异。在35例确诊和1例可能为进行性多灶性白质脑病的病例中发现了含乳头多瘤空泡病毒抗原的细胞，主要为PML细胞。其中29例脑与抗JCV血清发生反应。在5例脑中，PML病变某些区域的星形胶质细胞胞质和突起中有明显的抗原沉积。6例中巨大星形胶质细胞显示病毒抗原，表明这些细胞在进行性多灶性白质脑病中确实受到感染。神经细胞和内皮细胞无染色。
