Vasil'ev Iu M, Liubimov A V
Tsitologiia. 1983 Mar;25(3):283-9.
Distribution of fibronectin on the surface of lamelloplasm and endoplasm of single cultured mouse embryo fibroblasts was studied by means of indirect immunofluorescence technique. Large fibronectin fibrillae were located on the surface of endoplasm mainly, while "spots" and "streaks" of fibronectin dominated on the surface of lamelloplasm. Colcemid-treated cells had approximately the same distribution whereas cytochalasin B-treated cells were characterized by a spotty fluorescence alone. In contrast, in dense cultures of colcemid-treated cells fibronectin network underwent tearing, while it remained unchanged in cytochalasin B-treated cells. The centripetal movement of the fibronectin--receptor complexes along the cell surface from lamelloplasm into endoplasm (or the natural capping of fibronectin receptors) seems to play an important role in the assembling of fibronectin fibrillae.
采用间接免疫荧光技术研究了纤连蛋白在单层培养的小鼠胚胎成纤维细胞的片状伪足和内质表面的分布。大型纤连蛋白纤维主要位于内质表面，而纤连蛋白的“斑点”和“条纹”则在片状伪足表面占主导。秋水仙酰胺处理的细胞具有大致相同的分布，而细胞松弛素B处理的细胞仅表现为斑点状荧光。相反，在秋水仙酰胺处理的细胞密集培养物中，纤连蛋白网络发生撕裂，而在细胞松弛素B处理的细胞中则保持不变。纤连蛋白-受体复合物沿细胞表面从片状伪足向内质的向心运动（或纤连蛋白受体的天然帽化）似乎在纤连蛋白纤维的组装中起重要作用。
