Liubimov A V
Biull Eksp Biol Med. 1984 Jan;97(1):74-6.
Indirect immunofluorescence was used to study the distribution of surface fibronectin in normal embryonic and in four lines of transformed mouse fibroblasts. Accumulation of the fibronectin matrix with increasing culture density was discovered not only in normal but also in transformed cells. In three out of the four transformed lines studied, fibronectin was found in a considerable amount, which is in agreement with the data obtained with lactoperoxidase iodination. In these neoplastic lines, fibronectin was organized in the form of a fibrillar network like in normal cells. The data provided indicate that the changes in fibronectin content upon neoplastic transformation should be evaluated in dense cultures with maximum expression of this glycoprotein.
采用间接免疫荧光法研究正常胚胎及四株转化小鼠成纤维细胞系表面纤连蛋白的分布。不仅在正常细胞中，而且在转化细胞中都发现随着培养密度的增加纤连蛋白基质会积累。在所研究的四株转化细胞系中的三株中，发现有相当数量的纤连蛋白，这与用乳过氧化物酶碘化法获得的数据一致。在这些肿瘤细胞系中，纤连蛋白像在正常细胞中一样以纤维状网络的形式存在。所提供的数据表明，肿瘤转化时纤连蛋白含量的变化应在该糖蛋白表达最大化的致密培养物中进行评估。
