Döller G, Gröner A, Straub O C
Appl Environ Microbiol. 1983 Apr;45(4):1229-33. doi: 10.1128/aem.45.4.1229-1233.1983.
To evaluate the hygienic risk involved in using baculoviruses for insect pest control, safety studies are required. Pigs were chosen as representative test animals of commercial and agricultural importance. The tests were aimed at detecting virus propagation, immune reactions, and signs of acute infection (changes in body temperature and hematology profile, swelling of lymph nodes). Four of five animals inoculated with nuclear polyhedrosis virus showed a slight temperature rise at day 2 postinfection. After day 4 postinfection, no differences between infected animals and controls were observed. In the bioassay, virus activity could be recovered from fecal samples; however, no activity was found in organ extracts. The data did not indicate hygienic risks involved in the application of nuclear polyhedrosis virus, especially that from Mamestra brassicae, in biological pest control.
为评估使用杆状病毒进行害虫防治所涉及的卫生风险，需要开展安全性研究。猪被选为具有商业和农业重要性的代表性试验动物。这些试验旨在检测病毒繁殖、免疫反应以及急性感染迹象（体温和血液学指标变化、淋巴结肿大）。接种核型多角体病毒的五只动物中有四只在感染后第2天出现轻微体温升高。感染后第4天之后，未观察到感染动物与对照之间存在差异。在生物测定中，可从粪便样本中回收病毒活性；然而，在器官提取物中未发现活性。数据未表明在生物害虫防治中应用核型多角体病毒，尤其是来自甘蓝夜蛾的核型多角体病毒涉及卫生风险。
