Haskell C M, Buchegger F, Schreyer M, Carrel S, Mach J P
Cancer Res. 1983 Aug;43(8):3857-64.
As part of an ongoing effort to improve the technique of immunoscintigraphy for the detection of human carcinomas with radiolabeled monoclonal antibodies (MABs) to carcinoembryonic antigen (CEA), we have developed a series of MABs to CEA and have studied the effects of low- and physiological molarity buffers on their CEA binding and affinity, as well as their cross-reactivity with granulocyte glycoprotein(s). These in vitro results in different buffer systems were then correlated with the use of these MABs to CEA in the detection of human colon carcinoma grafts in nude mice. Our results show that the binding of CEA by some MABs is influenced by ionic strength and that this may be an important factor in their successful use for the immunolocalization of carcinomas in vivo.
作为持续努力改进用放射性标记的抗癌胚抗原（CEA）单克隆抗体（MABs）检测人类癌症的免疫闪烁成像技术的一部分，我们已研发出一系列抗CEA的MABs，并研究了低摩尔浓度和生理摩尔浓度缓冲液对其CEA结合力和亲和力的影响，以及它们与粒细胞糖蛋白的交叉反应性。然后，将这些在不同缓冲系统中的体外实验结果与使用这些抗CEA的MABs检测裸鼠体内人结肠癌移植瘤的情况相关联。我们的结果表明，某些MABs与CEA的结合受离子强度影响，这可能是它们在体内成功用于癌症免疫定位的一个重要因素。
