哺乳动物中链水解酶与酵母脂肪酸合成酶的相互作用。

The interaction of mammalian medium-chain hydrolase with yeast fatty acid synthetase.

作者信息

Slabas A R, Ormesher J, Roberts P A, Sidebottom C M, Tombs M P, Jeffcoat R, James A T

出版信息

Eur J Biochem. 1983 Jul 15;134(1):27-32. doi: 10.1111/j.1432-1033.1983.tb07526.x.

DOI:10.1111/j.1432-1033.1983.tb07526.x

Abstract

The interaction of rat mammary gland medium-chain thioesterase with yeast fatty acid synthetase has been investigated. Medium-chain thioesterase interacts with yeast fatty acid synthetase causing premature chain termination of the fatty acids synthesized from acetyl-CoA and malonyl-CoA. This effect is most marked under conditions of rate-limiting malonyl-CoA availability. Immobilized yeast fatty acid synthetase specifically binds rat mammary gland medium-chain thioesterase. This interaction has been used to purify medium-chain thioesterase to near homogeneity from samples of rat mammary gland cytosol. The stoichiometry of binding of medium-chain thioesterase to yeast fatty acid synthetase has been investigated. Yeast fatty acid synthetase binds 5.7 +/- 1 mol medium-chain thioesterase/mol yeast fatty acid synthetase. It is concluded that yeast fatty acid synthetase has a medium-chain thioesterase binding site.

摘要

已对大鼠乳腺中链硫酯酶与酵母脂肪酸合成酶的相互作用进行了研究。中链硫酯酶与酵母脂肪酸合成酶相互作用，导致由乙酰辅酶A和丙二酰辅酶A合成的脂肪酸提前链终止。在丙二酰辅酶A供应限速的条件下，这种效应最为明显。固定化酵母脂肪酸合成酶特异性结合大鼠乳腺中链硫酯酶。这种相互作用已被用于从大鼠乳腺细胞溶胶样品中将中链硫酯酶纯化至接近均一。已研究了中链硫酯酶与酵母脂肪酸合成酶结合的化学计量关系。酵母脂肪酸合成酶每摩尔结合5.7±1摩尔中链硫酯酶/摩尔酵母脂肪酸合成酶。得出的结论是，酵母脂肪酸合成酶具有一个中链硫酯酶结合位点。