泌乳兔乳腺中中链脂肪酸的合成。中链酰基硫酯水解酶的细胞内浓度及特异性。
Medium-chain fatty acid synthesis in lactating-rabbit mammary gland. Intracellular concentration and specificity of medium-chain acyl thioester hydrolase.
作者信息
Knudsen J
出版信息
Biochem J. 1979 Aug 1;181(2):267-74. doi: 10.1042/bj1810267.
The concentration of medium-chain acyl thioester hydrolase and of fatty acid synthetase was determined by rocket immunoelectrophoresis in nine different particle-free supernatant fractions from lactating-rabbit mammary gland. The molar ratio of the hydrolase to fatty acid synthetase was 1.99 +/- 0.66 (mean +/- S.D.). A rate-limiting concentration of malonyl-CoA was required to ensure the predominant synthesis of medium-chain fatty acids when 2 mol of the hydrolase was added per mol of fatty acid synthetase. The interaction of the hydrolase with fatty acid synthetase was concentration-dependent, though an optimum concentration of hydrolase to synthetase could not be obtained. The lactating-rabbit mammary gland hydrolase altered the pattern of fatty acids synthesized by fatty acid synthetases prepared from cow, goat, sheep and rabbit lactating mammary glands, rabbit liver and cow adipose tissue.
通过火箭免疫电泳法测定了来自泌乳兔乳腺的九个不同无颗粒上清液组分中中链酰基硫酯水解酶和脂肪酸合成酶的浓度。水解酶与脂肪酸合成酶的摩尔比为1.99±0.66（平均值±标准差）。当每摩尔脂肪酸合成酶添加2摩尔水解酶时，需要丙二酰辅酶A的限速浓度以确保主要合成中链脂肪酸。水解酶与脂肪酸合成酶的相互作用是浓度依赖性的，尽管无法获得水解酶与合成酶的最佳浓度。泌乳兔乳腺水解酶改变了由牛、山羊、绵羊和兔泌乳乳腺、兔肝脏和牛脂肪组织制备的脂肪酸合成酶所合成的脂肪酸模式。
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