Warenius H M, Taylor J W, Durack B E, Cross P A
Eur J Cancer Clin Oncol. 1983 Mar;19(3):347-55. doi: 10.1016/0277-5379(83)90132-3.
Human immunoglobulin-secreting hybridomas have been produced by fusion of the LICR-LON-HMy2 line with lymph node lymphocytes from patients with malignant melanoma. Immunoglobulin production as measured by reverse haemolytic plaque assay was greater for hybridomas than for the parent HMy2 line. Pre-incubation of unseparated cultures from lymph nodes with pokeweed mitogen resulted in more successful fusions with a greater yield of hybridomas. The hybrids did not, however, have any greater immunoglobulin production than did those from lymphocytes not pre-incubated with pokeweed mitogen. Using lymph node cells from a patient with malignant melanoma of the cervical region, a consistent pattern of increased immunoglobulin binding to three human melanoma cell lines in contrast to a human colonic adenocarcinoma and a human skin-derived fibroblast line was demonstrated.
通过将LICR-LON-HMy2细胞系与恶性黑色素瘤患者的淋巴结淋巴细胞融合，已产生了分泌人免疫球蛋白的杂交瘤。通过反向溶血空斑试验测定，杂交瘤产生的免疫球蛋白比亲本HMy2细胞系更多。用商陆有丝分裂原对未分离的淋巴结培养物进行预孵育，可使融合更成功，杂交瘤产量更高。然而，与未用商陆有丝分裂原预孵育的淋巴细胞产生的杂交瘤相比，这些杂交瘤产生的免疫球蛋白并没有更多。使用来自一名颈部恶性黑色素瘤患者的淋巴结细胞，证实了与人类结肠腺癌和人类皮肤来源的成纤维细胞系相比，免疫球蛋白与三种人类黑色素瘤细胞系结合增加的一致模式。
