通过蛋白A反向溶血空斑试验评估犬B淋巴细胞的多克隆激活。
Polyclonal activation of canine B lymphocytes evaluated by a protein A reverse hemolytic plaque assay.
作者信息
Felsburg P J, Serra D A, Mandato V N, Jezyk P F
出版信息
Vet Immunol Immunopathol. 1984 Jul;6(3-4):353-64. doi: 10.1016/0165-2427(84)90060-6.
This paper describes the optimal culture and assay conditions for the polyclonal activation of canine lymphocytes with pokeweed mitogen and the quantitation of immunoglobulin secreting plaque-forming cells (PFC) using a staphylococcal protein A-reverse hemolytic plaque assay. The assay permits the quantitation of total immunoglobulin secreting PFC as well as class-specific immunoglobulin secreting PFC. On the optimal day of culture, a mean of 176 IgA PFC/10(6), 575 IgM PFC/10(6), 1276 IgG PFC/10(6), and 2158 total PFC/10(6) cells were generated following polyclonal activation. This study provides a simple and reproducible assay for the delineation of the immunoregulatory mechanisms involved in the differentiation of canine B lymphocytes.
本文描述了用商陆有丝分裂原对犬淋巴细胞进行多克隆激活的最佳培养和检测条件，以及使用葡萄球菌蛋白A反向溶血空斑试验对免疫球蛋白分泌性空斑形成细胞（PFC）进行定量分析。该试验可对总免疫球蛋白分泌性PFC以及类特异性免疫球蛋白分泌性PFC进行定量分析。在培养的最佳天数，多克隆激活后平均产生176个IgA PFC/10⁶、575个IgM PFC/10⁶、1276个IgG PFC/10⁶和2158个总PFC/10⁶细胞。本研究提供了一种简单且可重复的试验方法，用于描绘犬B淋巴细胞分化过程中涉及的免疫调节机制。
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