Soda R, Tavassoli M
Exp Cell Res. 1983 May;145(2):389-95. doi: 10.1016/0014-4827(83)90017-4.
The distribution of insulin receptors was studied in rat liver cell suspensions using a latex minibead covalently bound to insulin. This probe can be visualized by electron microscopy (EM). Using this visual probe, the highest density of the receptor was found on endothelial cells in the cell suspension, with hepatocytes having only few receptors and Kupffer cells having none. Fractionation of liver cell suspensions on metrizamide gradients yielded two populations of cells; large cells (hepatocytes) and small cells which consisted mostly of Kupffer cells and endothelial cells, distinguishable by their surface and cytoplasmic features. Again, by the use of an insulin-minibead probe, the highest density of receptors was found on endothelial cells. It is suggested that the endothelium has a crucial role in the uptake and transport of the hormone across the tissue-blood barrier.
利用与胰岛素共价结合的乳胶微珠，对大鼠肝细胞悬液中的胰岛素受体分布进行了研究。该探针可通过电子显微镜（EM）观察到。使用这种可视化探针，在细胞悬液中的内皮细胞上发现了最高密度的受体，肝细胞仅有少量受体，而库普弗细胞则没有受体。在甲泛葡胺梯度上对肝细胞悬液进行分级分离，得到了两类细胞群体；大细胞（肝细胞）和小细胞，小细胞主要由库普弗细胞和内皮细胞组成，可通过其表面和细胞质特征区分开来。同样，通过使用胰岛素微珠探针，在内皮细胞上发现了最高密度的受体。这表明内皮细胞在激素跨组织 - 血屏障的摄取和转运中起关键作用。
