Takahashi K, Tavassoli M
J Ultrastruct Res. 1983 Jun;83(3):233-41. doi: 10.1016/s0022-5320(83)90131-4.
In monolayer cultures, adipocytes transform into spindle-shaped cells, morphologically similar to buccal submucosa-derived fibroblasts. Insulin receptors were searched for on the surface of these cells using a visual probe which consisted of latex minibeads covalently bound to insulin. Adipocyte-derived cells showed clusters of insulin receptors not observed on the surface of fibroblasts derived from submucosa. The finding indicates that despite their fibroblastic morphology and the loss of their lipid inclusions in culture, these adipocytes do not lose their state of differentiation and therefore should be considered lipid-depleted adipocytes rather than fibroblasts. Moreover, quantitative analysis demonstrated a significant increase in the number of insulin receptors in cultured cells as compared to isolated but not cultured cells. This may reflect the low concentration of insulin in the culture medium as compared to the in vivo environment and indicates that the cells are subject to regulatory mechanisms of adipocytes.
在单层培养中，脂肪细胞转变为纺锤形细胞，形态上类似于颊黏膜下层来源的成纤维细胞。使用由与胰岛素共价结合的乳胶微珠组成的视觉探针，在这些细胞表面寻找胰岛素受体。脂肪细胞来源的细胞显示出成纤维细胞表面未观察到的胰岛素受体簇。这一发现表明，尽管它们具有成纤维细胞形态且在培养中失去了脂质包涵体，但这些脂肪细胞并未失去其分化状态，因此应被视为脂质耗尽的脂肪细胞而非成纤维细胞。此外，定量分析表明，与分离但未培养的细胞相比，培养细胞中的胰岛素受体数量显著增加。这可能反映了与体内环境相比，培养基中胰岛素浓度较低，并表明这些细胞受到脂肪细胞调节机制的影响。
