丁酸梭菌氢化酶基因在大肠杆菌中的克隆与表达。
Cloning and expression of the hydrogenase gene from Clostridium butyricum in Escherichia coli.
作者信息
Karube I, Urano N, Yamada T, Hirochika H, Sakaguchi K
出版信息
FEBS Lett. 1983 Jul 11;158(1):119-22. doi: 10.1016/0014-5793(83)80689-9.
PMID:6345200
Abstract
Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3.1-3.5-times as high as those of the present strains, such as C.butyricum and E.coli C600 (Hyd+).
摘要
使用pBR322和PstI，将丁酸梭菌的氢化酶基因克隆到大肠杆菌HK16（Hyd-）中。含有氢化酶基因的质粒pCBH1大小为7.3 MDa，pCBH1有5个PstI-DNA片段（分别为3.9、2.6、0.7、0.03 - 0.04、小于0.02 MDa）。HK16（pCBH1）的氢化酶活性约为本菌株（如丁酸梭菌和大肠杆菌C600（Hyd+））的3.1 - 3.5倍。
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