Ascher N L, Chen S, Hoffman R A, Simmons R L
J Immunol. 1983 Aug;131(2):617-21.
Two parallel events may provide cytotoxic effector cells at the site of allograft challenge: 1) the migration of effector cells to the graft, and 2) the local maturation of effector cells. Various experimental conditions were manipulated to ascertain the relative role of local maturation of precytotoxic cells at the site of alloantigen challenge. A C57BL/6 allogeneic-coated sponge matrix allograft was placed in a BALB/c animal. The entire animal with the sponge was subjected to sublethal irradiation, or alternatively, just the animal was subjected to the same dose of irradiation, and the sponge was shielded. The data demonstrate that by day 5, precytotoxic cells directed against donor alloantigen appears within the sponge matrix allograft. These precytotoxic cells are in themselves x-ray sensitive, but in the absence of irradiation and in the absence of further contributions from the host can develop into mature cytotoxic cells with specificity limited to that of donor alloantigen. The relative role of local maturation vs continued influx of mature cytotoxic cells cannot be determined from these sets of experiments. These data, however, show that there is potential for local development of mature cytotoxic cells in the absence of other host influences.
1）效应细胞向移植物的迁移，以及2）效应细胞的局部成熟。通过操纵各种实验条件来确定前细胞毒性细胞在同种异体抗原攻击部位局部成熟的相对作用。将C57BL/6同种异体包被的海绵基质同种异体移植物植入BALB/c动物体内。对带有海绵的整个动物进行亚致死剂量照射，或者仅对动物进行相同剂量照射，而对海绵进行屏蔽。数据表明，到第5天，针对供体同种异体抗原的前细胞毒性细胞出现在海绵基质同种异体移植物内。这些前细胞毒性细胞本身对X射线敏感，但在没有照射且没有宿主进一步贡献的情况下，可以发育成成熟的细胞毒性细胞，其特异性仅限于供体同种异体抗原。从这些实验中无法确定局部成熟与成熟细胞毒性细胞持续流入的相对作用。然而，这些数据表明，在没有其他宿主影响的情况下，成熟细胞毒性细胞存在局部发育的可能性。
