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用于检测抗弓形虫抗体的酶联免疫吸附测定(ELISA)

[Enzyme-linked-immunosorbent assay (ELISA) for demonstrating antibodies against toxoplasmosis].

作者信息

Walter G H, Manthey K F

出版信息

Klin Monbl Augenheilkd. 1983 Apr;182(4):314-7. doi: 10.1055/s-2008-1054774.

Abstract

Serologic tests for specific antibodies are the primary method of diagnosis in suspected cases of toxoplasmosis. Available tests differ widely with regard to specificity and sensitivity. The enzyme-linked immunosorbent assay (ELISA) is specific for toxoplasmosis and has a sensitivity comparable to radio-immuno assays. The reproducibility of the CORDIA-T assay, tested by the authors in their laboratory, was excellent; the test itself was very easy to perform. When testing sera of 590 apparently normal subjects the authors found specific antibodies in 17.9% to 48.1% of all cases, depending on age. The ELISA-test seems to be useful in the diagnosis of ocular toxoplasmosis.

摘要

针对特定抗体的血清学检测是疑似弓形虫病病例的主要诊断方法。现有检测在特异性和敏感性方面差异很大。酶联免疫吸附测定(ELISA)对弓形虫病具有特异性,其敏感性与放射免疫测定相当。作者在其实验室测试的CORDIA - T测定的可重复性极佳;该测试本身非常易于操作。在检测590名明显正常受试者的血清时,作者发现根据年龄不同,所有病例中有17.9%至48.1%存在特异性抗体。ELISA检测似乎对眼弓形虫病的诊断有用。

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