Brocchi E, Genzini E, Valcavi P, Schito G C
Boll Ist Sieroter Milan. 1981;60(6):498-507.
The enzyme-linked immunosorbent assay (ELISA) has been applied for detecting anti-toxoplasma serum IgG and IgM. ELISA was carried out with alkaline phosphatase-labeled anti-IgG and anti-IgM antibodies. Both conjugates specificity was investigated by sucrose density gradient fractionating of an immunofluorescence positive serum and by ELISA testing of all the fractions for IgG and IgM. A good agreement of ELISA results from 400 sera was found when they were compared with others obtained by several serological methods: direct agglutination, complement fixation, IgG/IgM-IIF. Occasionally false-positive IgM-ELISA and IgM-IIF results were observed, probably due to non specific IgM antibodies. Absorption of sera with whole parasites resulted in a removal of specific IgM, whilst false-positive IgM-ELISA reactions were unaffected. ELISA procedure is simple and rapid to carry out in a large scale and it seems to be for routine purposes an adequate substitute for toxoplasmosis IIF test. The possibility to quantitate ELISA results would give the opportunity to standardize the test not only in one, but even among several laboratories.
酶联免疫吸附测定（ELISA）已用于检测抗弓形虫血清IgG和IgM。采用碱性磷酸酶标记的抗IgG和抗IgM抗体进行ELISA检测。通过对免疫荧光阳性血清进行蔗糖密度梯度分级，并对所有分级进行IgG和IgM的ELISA检测，研究了两种结合物的特异性。将400份血清的ELISA结果与通过几种血清学方法（直接凝集、补体结合、IgG/IgM-IIF）获得的其他结果进行比较时，发现结果吻合良好。偶尔会观察到假阳性的IgM-ELISA和IgM-IIF结果，可能是由于非特异性IgM抗体所致。用完整寄生虫对血清进行吸收可去除特异性IgM，而假阳性的IgM-ELISA反应不受影响。ELISA方法操作简单、可大规模快速进行，似乎可作为弓形虫病IIF检测的常规合适替代方法。定量ELISA结果的可能性不仅能使该检测在一个实验室，甚至在多个实验室之间实现标准化。
