Sigel M B, Sinha Y N, VanderLaan W P
Methods Enzymol. 1983;93:3-12. doi: 10.1016/s0076-6879(83)93031-8.
The intra-lymph node technique used to inoculate rabbits with small quantities of antigen has been described. A variety of antigens in the 20,000-22,000 molecular weight range, as well as a 15-amino acid peptide coupled to BSA, have been inoculated successfully by this procedure. We have made no attempt to compare the success rate of the intra-lymph node inoculation route with other techniques utilizing small (microgram) quantities of antigen.
已描述了用于给兔子接种少量抗原的淋巴结内技术。通过该程序已成功接种了多种分子量在20,000 - 22,000范围内的抗原,以及与牛血清白蛋白偶联的一种15氨基酸肽。我们没有尝试将淋巴结内接种途径的成功率与其他使用少量(微克)抗原的技术进行比较。