Fazly Bazaz B S, Salt W G
Microbios. 1983;36(145-46):135-47.
Four local anaesthetics (LAs), amethocaine and procaine (esters) and cinchocaine and lignocaine (amides) have been assessed in terms of their ability to induce turbidity increases in non-growing cells of Escherichia coli suspended in a carbohydrate free defined medium (CFM) or distilled water or in 0.2 M phosphate buffer. Procaine and lignocaine did not induce turbidity increases. The turbidity increase-LA concentration profiles for both amethocaine and cinchocaine are similar in all three systems. Slight variation are discussed in terms of the ionic content of the suspending media. Interactions between LAs and dispersed systems comprising: cell envelope preparations, cytoplasmic contents, lipid depleted cells and cell lipid dispersion, are also described. Transmission electron micrographs of LA treated cells are presented and the use of uranyl acetate as a 'vital' stain is illustrated. Results are discussed in terms of LA lipophilicity and the effectiveness of LAs in precipitating intracellular materials at high concentrations.
已对四种局部麻醉剂（LAs），即丁卡因和普鲁卡因（酯类）以及辛可卡因和利多卡因（酰胺类）进行了评估，评估内容为它们在无碳水化合物限定培养基（CFM）、蒸馏水或0.2M磷酸盐缓冲液中悬浮的非生长型大肠杆菌细胞中引起浊度增加的能力。普鲁卡因和利多卡因未引起浊度增加。在所有三种体系中，丁卡因和辛可卡因的浊度增加-LA浓度曲线相似。根据悬浮介质的离子含量讨论了细微差异。还描述了局部麻醉剂与以下分散体系之间的相互作用：细胞膜制剂、细胞质内容物、脂质耗尽细胞和细胞脂质分散体。展示了经局部麻醉剂处理的细胞的透射电子显微镜照片，并说明了乙酸铀酰作为“活体”染色剂的使用。根据局部麻醉剂的亲脂性以及局部麻醉剂在高浓度下沉淀细胞内物质的有效性对结果进行了讨论。
