Messiha F S, Price J
Neurobehav Toxicol Teratol. 1983 Mar-Apr;5(2):251-4.
The rat eye possesses cytoplasmic aldehyde dehydrogenase (ALDH) activity. The enzyme is present in whole eye, in ocular fluids and is distributed in specific ocular regions. The specific activity of cornea ALDH was the highest among 6 regions studied. The ciliary body and iris combined displayed second largest activity followed by even distribution of ALDH in the retina, the sclera and the surrounding connective tissue. The cloroid and lens did not show measurable ALDH activity. The kinetics property of cornea-ALDH indicate high Vmax. In vitro addition of equal protein concentration of individual ocular regional preparations to the cornea decreased specific activity of cornea-ALDH suggesting of protein interaction and/or substrate competition phenomenon. Short-term oral administration of Vitamin A resulted in statistically insignificant increase of whole ocular tissue ALDH from controls as contrasted with inhibition of ALDH in the ocular fluid by similar short-term oral administration of disulfiram.
大鼠眼具有细胞质醛脱氢酶（ALDH）活性。该酶存在于全眼、眼内液中，并分布于特定的眼部区域。在所研究的6个区域中，角膜ALDH的比活性最高。睫状体和虹膜的组合显示出第二大活性，其次是视网膜、巩膜和周围结缔组织中ALDH的均匀分布。脉络膜和晶状体未显示出可测量的ALDH活性。角膜ALDH的动力学特性表明其Vmax较高。在体外将各眼部区域制剂等蛋白浓度添加到角膜中，会降低角膜ALDH的比活性，提示存在蛋白质相互作用和/或底物竞争现象。与短期口服双硫仑抑制眼内液中的ALDH相比，短期口服维生素A导致全眼组织ALDH较对照组有统计学上不显著的增加。
