Takeuchi Y, Matsuura T, Yonezawa T, Sano Y
Arch Histol Jpn. 1983 Feb;46(1):79-86. doi: 10.1679/aohc.46.79.
Serotonin neurons in newborn mouse and rat brainstem cultures were studied by peroxidase-antiperoxidase immunohistochemistry using a serotonin antibody. The intensity of the immunohistochemical reaction in the neuronal somata decreased gradually with time and was hardly detectable after one month of culturing. On the other hand, the immunoreactivity of the processes noted at the early stages later on became more intense and covered the full extent of the fibers. From the early stages of the cultures, the axon of each serotonin neuron formed not only a network by branching, but also a true anastomosis with the axonal networks of the other serotonin neurons.
利用血清素抗体，通过过氧化物酶-抗过氧化物酶免疫组织化学方法对新生小鼠和大鼠脑干培养物中的血清素神经元进行了研究。神经元胞体中免疫组织化学反应的强度随时间逐渐降低，培养一个月后几乎检测不到。另一方面，早期观察到的突起的免疫反应性后来变得更强，并覆盖了纤维的全长。从培养早期开始，每个血清素神经元的轴突不仅通过分支形成网络，还与其他血清素神经元的轴突网络形成真正的吻合。
