Ihara N, Nishida K, Ueda S, Kawata M, Sano Y
Neurosci Res. 1985 Dec;3(2):145-53. doi: 10.1016/0168-0102(85)90027-6.
A peroxidase-antiperoxidase immunohistochemical method with serotonin antiserum was employed to investigate the influence of reserpine on serotonin neurons of rats which were sacrificed at various times after injection (10 mg/kg i.p.). The disappearance of serotonin immunoreactivity induced by reserpine was detected only in the perikarya after 15 min, and then rapidly proceeded to the terminals. Between 2 and 4 h, immunoreactivity completely disappeared throughout the brain. The immunoreactivity reappeared in the perikarya after 6 h, and progressed toward the terminals gradually. However, there was an obvious difference in the rate of recovery of immunoreactivity between areas. After 7 days, the immunoreactivity returned to control levels.
采用过氧化物酶 - 抗过氧化物酶免疫组织化学方法，用血清素抗血清研究利血平对大鼠血清素神经元的影响，这些大鼠在腹腔注射（10mg/kg）后不同时间被处死。利血平诱导的血清素免疫反应性消失仅在注射后15分钟时在胞体中被检测到，然后迅速发展到终末。在2至4小时之间，整个大脑中的免疫反应性完全消失。免疫反应性在6小时后在胞体中重新出现，并逐渐向终末发展。然而，不同区域之间免疫反应性的恢复速率存在明显差异。7天后，免疫反应性恢复到对照水平。
