Strugnell R A, Underwood J R, Clarke F M, Pedersen J S, Chalmers P J, Faine S, Toh B H
Clin Exp Immunol. 1983 Jun;52(3):537-42.
A monoclonal IgM smooth muscle antibody secreted by a hybrid (MMI-1) of mouse plasmacytoma NS-1 with spleen cells from mouse immunized with Treponema pallidum was detected by indirect immunofluorescence tests on frozen tissue sections and on acetone fixed monolayers of rat and human fibroblasts. The antibody did not react with acetone fixed smears of T. pallidum but reacted with smooth muscle fibres and with striations of skeletal and cardiac muscle. In non-muscle cells, the antibody stained liver in a 'polygonal' pattern, thymus with accentuated staining of the thymic medulla, renal glomeruli and the brush border and peritubular fibrils of renal tubules. In fibroblast monolayers, the antibody stained stress fibres in an interrupted pattern. Immunoblotting with muscle proteins and the antibody showed labelling of a 100K molecule. The cellular distribution of the mouse monoclonal antibody is similar to that obtained with anti-actin antibody suggesting that the corresponding antigen may be an actin binding protein.
通过对冷冻组织切片以及大鼠和人成纤维细胞的丙酮固定单层细胞进行间接免疫荧光试验，检测到一种由小鼠浆细胞瘤NS - 1与用梅毒螺旋体免疫的小鼠脾脏细胞杂交产生的单克隆IgM平滑肌抗体（MMI - 1）。该抗体不与梅毒螺旋体的丙酮固定涂片发生反应，但与平滑肌纤维以及骨骼肌和心肌的条纹发生反应。在非肌肉细胞中，该抗体以“多边形”模式染色肝脏，胸腺的髓质染色增强，肾小体以及肾小管的刷状缘和肾小管周围纤维。在成纤维细胞单层中，该抗体以间断模式染色应力纤维。用肌肉蛋白和该抗体进行免疫印迹显示标记了一个100K的分子。小鼠单克隆抗体的细胞分布与用抗肌动蛋白抗体获得的分布相似，表明相应抗原可能是一种肌动蛋白结合蛋白。
