Steiner B, McLean I, Graves S
Br J Vener Dis. 1981 Oct;57(5):295-301. doi: 10.1136/sti.57.5.295.
A strongly reduced culture medium, capable of maintaining the virulence of Treponema pallidum (Nichols) for several days, was exposed to an atmosphere of 3% oxygen in nitrogen for 2-3 days before inoculation with T pallidum. By using various volumes of medium in uniform tubes a range of redox potentials (Ecal) from -94 mV to -325 mV was produced depending on the surface area-to-volume ratios of the medium. The anaerobic medium had an Ecal value of -387 mV. The medium was inoculated with T pallidum and incubated in an atmosphere of 3% oxygen. The survival of treponemes at different redox potentials was monitored by observing the retention of motility and by measuring the latent period of infection after inoculation of the cultures into the shaved backs of rabbits. Under these conditions T pallidum survived longest at low (electronegative) redox potential. An inverse linear relationship was observed between the redox potential of the culture medium and the survival of T pallidum, as measured by the time required for a 90% reduction of virulent organisms. No optimum redox potential was detected, the most electronegative medium (-325 mV, Ecal) giving the best survival.
一种能使梅毒螺旋体（Nichols株）毒力维持数日的高度稀释培养基，在接种梅毒螺旋体之前，先在含3%氧气的氮气环境中暴露2 - 3天。通过在规格一致的试管中使用不同体积的培养基，根据培养基的表面积与体积比，产生了从-94 mV到-325 mV的一系列氧化还原电位（Ecal）。厌氧培养基的Ecal值为-387 mV。将培养基接种梅毒螺旋体后，置于含3%氧气的环境中培养。通过观察螺旋体的运动能力以及测量将培养物接种到兔背部剃毛处后的感染潜伏期，来监测不同氧化还原电位下螺旋体的存活情况。在这些条件下，梅毒螺旋体在低（负电）氧化还原电位下存活时间最长。通过测量90%的有毒生物体减少所需时间来衡量，观察到培养基的氧化还原电位与梅毒螺旋体的存活之间呈反线性关系。未检测到最佳氧化还原电位，最负电的培养基（-325 mV，Ecal）使螺旋体存活得最好。
