Rodning C B, Erlandsen S L, Wilson I D, Carpenter A M
Dig Dis Sci. 1983 Aug;28(8):742-50. doi: 10.1007/BF01312566.
A light microscopic morphometric analysis of IgA-containing immunocytes within samples of ileal mucosa was performed. The following groups of rats were studied: (1) animals raised in a gnotobiotic environment (microbial reduction); (2) animals with iatrogenic self-filling intestinal blind loops (microbial proliferation); and (3) control animals (sham operation). The unlabeled antibody enzyme immunohistochemical localization technique was employed for the identification of intracellular IgA. Component quantitation involved use of a micrometer component quantitator. Numerical density of the immunocyte population was determined by component quantitation of individual and total immunocyte volumes and by application of the Floderus equation. The methodology employed provided a precise quantitative analysis of all mucosal components of normal and manipulated rat ileum. A statistically significant reduction in the volume percentage of IgA-containing immunocytes in association with both microbial reduction and microbial proliferation was observed. The volume percentage reduction of the IgA-containing immunocyte population associated with gnotobiosis may reflect decreased microbial antigenic stimulation, whereas that associated with microbial proliferation may reflect the presence of an increased population of immunocytes producing non-IgA immunoglobulins.
对回肠黏膜样本中含IgA免疫细胞进行了光镜形态计量分析。研究了以下几组大鼠：(1) 在悉生环境中饲养的动物（微生物减少）；(2) 有医源性自充盈肠盲袢的动物（微生物增殖）；(3) 对照动物（假手术）。采用未标记抗体酶免疫组织化学定位技术鉴定细胞内IgA。成分定量涉及使用测微计成分定量仪。免疫细胞群体的数值密度通过对单个和总免疫细胞体积进行成分定量并应用弗洛德勒斯方程来确定。所采用的方法对正常和经处理的大鼠回肠的所有黏膜成分进行了精确的定量分析。观察到与微生物减少和微生物增殖相关的含IgA免疫细胞体积百分比均有统计学意义的降低。与无菌生活相关的含IgA免疫细胞群体体积百分比降低可能反映微生物抗原刺激减少，而与微生物增殖相关的降低可能反映产生非IgA免疫球蛋白的免疫细胞群体增加。
