一种利用乳胶凝集反应检测人血清中抗透明带活性的新方法。

A new method for detection of anti-zona activity in human sera using latex agglutination reaction.

作者信息

Noda Y, Yamada I, Hayashi S, Takai I, Watanabe E

出版信息

Experientia. 1983 Aug 15;39(8):926-8. doi: 10.1007/BF01990442.

DOI:10.1007/BF01990442

Abstract

A slide latex test for detection of anti-zona pellucida activity in human sera was developed using a latex agglutination reaction. The latex reagent, made of polystyrene particles coated with solubilizing zona antigen(s), was found to give results comparable in sensitivity as well as specificity to those of the indirect immunofluorescence method as tested with anti-pig-zona antiserum and with human sera. Thus, the slide latex test was judged to be adequate for use instead of the indirect immunofluorescence technique.

摘要

利用乳胶凝集反应开发了一种用于检测人血清中抗透明带活性的玻片乳胶试验。由包被有可溶解的透明带抗原的聚苯乙烯颗粒制成的乳胶试剂，在用抗猪透明带抗血清和人血清进行测试时，其灵敏度和特异性结果与间接免疫荧光法相当。因此，玻片乳胶试验被判定足以替代间接免疫荧光技术使用。