用分离出的人卵进行异种免疫。
Heteroimmunization with isolated human ova.
作者信息
Takai I, Mori T, Noda Y, Nishimura T
出版信息
J Reprod Fertil. 1981 Jan;61(1):19-24. doi: 10.1530/jrf.0.0610019.
Rabbit antiserum raised against isolated human ova produced an intense immunofluorescence and a precipitate on the outer surface of human and porcine zonae pellucidae. The antiserum reacted with none of 22 human tissues nor with any of 4 body fluid components by immunofluorescence or immunodiffusion analysis, but the antiserum agglutinated AB erythrocytes after absorption with O erythrocytes. The anti-zona activity was achieved by higher titres for human than for porcine zonae. Immunofluorescence on porcine zonae was completely abolished by absorption with porcine ova, whereas a weak but definite fluorescence remained on human zonae. These findings indicate that the human zona pellucida consists of at least three distinct components; (1) a specific antigen(s) shared by human and porcine zonae, (2) an antigen(s) specific to human zonae, and (3) a non-specific antigen(s) associated with the blood group substances.
针对分离出的人卵母细胞产生的兔抗血清，在人及猪的透明带上产生了强烈的免疫荧光和沉淀。通过免疫荧光或免疫扩散分析，该抗血清与22种人体组织中的任何一种以及4种体液成分中的任何一种均无反应，但在用O型红细胞吸收后，该抗血清凝集了AB型红细胞。人透明带的抗透明带活性效价比猪透明带的更高。用猪卵母细胞吸收后，猪透明带上的免疫荧光完全消失，而人透明带上仍残留微弱但明确的荧光。这些发现表明，人透明带至少由三种不同成分组成：（1）人及猪透明带共有的一种或多种特异性抗原；（2）人透明带特有的一种或多种抗原；（3）与血型物质相关的一种或多种非特异性抗原。
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