Steplewski Z, Herlyn M, Blaszczyk M, Koprowski H
J Immunol Methods. 1983 Aug 12;62(1):73-8. doi: 10.1016/0022-1759(83)90112-6.
We have selected 4 murine monoclonal antibodies that specifically bind to Lewis type blood group haptens to establish an assay in which antigens present in human saliva are bound to polystyrene beads and then detected by monoclonal antibodies in radioimmunoassay or in peroxidase-antiperoxidase immunoassay. Two of these antibodies detect both the Lea and Leb determinants (Leab), 1 antibody detects only Lea, and 1 only Leb. All Lewis phenotypes (Lea-b-, Lea+b-, Lea-b+ and Lea+b+) are easily detectable in this assay. Of the 60 individuals tested, 1 was Lea+b+, 4 Lea-b-, 12 Lea+b- and 43 Lea-b+.
我们选择了4种特异性结合Lewis血型抗原决定簇的鼠单克隆抗体，以建立一种检测方法，即将人唾液中的抗原结合到聚苯乙烯珠上，然后在放射免疫测定或过氧化物酶-抗过氧化物酶免疫测定中用单克隆抗体进行检测。其中两种抗体可同时检测Lea和Leb决定簇（Leab），1种抗体仅检测Lea，另1种仅检测Leb。在该检测方法中，所有Lewis血型表型（Lea-b-、Lea+b-、Lea-b+和Lea+b+）都易于检测。在检测的60个人中，1人为Lea+b+，4人为Lea-b-，12人为Lea+b-，43人为Lea-b+。
