Rees R C, Platts A A
J Immunol Methods. 1983 Aug 12;62(1):79-85. doi: 10.1016/0022-1759(83)90113-8.
Using whole blood from normal subjects, we have observed natural killing of K562 cells in a 4 h 51Cr-release assay comparable with that shown by separated PBMC and whole blood depleted of serum components. Separated plasma was not toxic towards K562 targets, and failed to potentiate the level of PBMC cytotoxicity through ADCC. The presence of red blood cells did not influence natural killing. The natural cytotoxicity of whole blood was augmented by interferon and depressed by prostaglandins E1 and E2. Studies with appropriate control blood fractions show that cytotoxicity tests with whole blood provide results reflecting natural cell-mediated cytotoxicity.
使用正常受试者的全血，我们在4小时51铬释放试验中观察到对K562细胞的自然杀伤作用，这与分离的外周血单核细胞（PBMC）和去除血清成分的全血所显示的结果相当。分离的血浆对K562靶细胞无毒，并且不能通过抗体依赖的细胞介导的细胞毒性（ADCC）增强PBMC的细胞毒性水平。红细胞的存在不影响自然杀伤作用。全血的自然细胞毒性可被干扰素增强，而被前列腺素E1和E2抑制。对适当对照血液组分的研究表明，用全血进行的细胞毒性试验提供了反映自然细胞介导的细胞毒性的结果。
