Kowarski C R, Bado B, Shah S, Kowarski D, Kowarski A A
J Pharm Sci. 1983 Jun;72(6):692-3. doi: 10.1002/jps.2600720623.
The bioactivity of equal doses (by radioimmunoassay) of regular insulin and sodium insulin were measured in six preconditioned female mongrel hound dogs by a previously described bioassay. The hypoglycemic nadir of blood glucose induced by intravenous injection of the drugs was compared with the nadirs induced by a known amount of the international standard insulin. The level of blood glucose was monitored-instantaneously and continuously using a nonthrombogenic continuous glucose-monitoring system. The hypoglycemic effect of subcutaneously injected sodium insulin was compared with the effect of subcutaneously injected regular insulin. The two drugs were injected at 1-2-week intervals to seven dogs. While the bioactivity of intravenously administered sodium insulin was equal to the bioactivity of intravenously administered regular insulin, the hypoglycemic effect of subcutaneously injected sodium insulin differed from the effect of subcutaneously injected regular insulin. The blood glucose nadir after subcutaneous injection of regular insulin occurred 101 +/- 21 min after the injection. The nadir after subcutaneous injection of sodium insulin occurred 163 +/- 33 min after the injection.
采用先前描述的生物测定法，在6只经过预处理的雌性杂种猎犬中测量了等量（通过放射免疫测定）的普通胰岛素和胰岛素钠的生物活性。将静脉注射药物诱导的血糖低血糖最低点与已知量的国际标准胰岛素诱导的最低点进行比较。使用非血栓形成的连续血糖监测系统即时且连续地监测血糖水平。比较了皮下注射胰岛素钠与皮下注射普通胰岛素的降血糖效果。将这两种药物每隔1 - 2周注射给7只狗。虽然静脉注射胰岛素钠的生物活性与静脉注射普通胰岛素的生物活性相等，但皮下注射胰岛素钠的降血糖效果与皮下注射普通胰岛素的效果不同。皮下注射普通胰岛素后的血糖最低点出现在注射后101±21分钟。皮下注射胰岛素钠后的最低点出现在注射后163±33分钟。
