Stevenson R W, Tsakok T I, Parsons J A
Diabetologia. 1980 May;18(5):423-6. doi: 10.1007/BF00276825.
A new technique of programmed intravenous insulin infusion at a series of decreasing rates has been used to imitate the magnitude and time course of biological responses obtained by the subcutaneous route. Groups of normal rats prepared with indwelling venous cannulae were injected subcutaneously with soluble porcine insulin, 0.4 U/kg. The pattern of the resulting hypoglycaemic response was subsequently matched by a 2-hour intravenous insulin infusion at rates decreasing stepwise from 0.3 to 0.05 U kg-1h-1. The total amount of insulin infused intravenously was only 50% of that required subcutaneously. In addition, subcutaneous or intravenous infusions of insulin at 0.05 U kg-1h-1 were given to two groups of rats from the same batch. When both infusions were continued until plateau responses were reached, a significantly greater lowering of plasma glucose was caused by the intravenous route. These results suggest that when insulin is given subcutaneously significant inactivation of the insulin occurs at or near the injection site.
一种采用一系列递减速率进行静脉内胰岛素程序化输注的新技术，已被用于模拟经皮下途径获得的生物学反应的强度和时间进程。给留置静脉插管的正常大鼠组皮下注射0.4 U/kg的可溶性猪胰岛素。随后，通过以从0.3至0.05 U kg-1h-1逐步递减的速率进行2小时静脉内胰岛素输注，来匹配所产生的低血糖反应模式。静脉内输注的胰岛素总量仅为皮下所需量的50%。此外，给来自同一批次的两组大鼠分别以0.05 U kg-1h-1的速率进行皮下或静脉内胰岛素输注。当两种输注都持续至达到平台反应时，静脉途径导致的血浆葡萄糖降低幅度明显更大。这些结果表明，当胰岛素经皮下给药时，胰岛素在注射部位或其附近会发生显著的失活。
