Eaton D L, Toal B F
Sci Total Environ. 1983 Jun;28:375-84. doi: 10.1016/s0048-9697(83)80034-5.
A new method was modified and evaluated as a simple, sensitive and quantitative procedure for measuring metallothionein (MT) in tissues. Heat denatured cytosolic fractions of tissue homogenates were incubated with 109Cd and bovine hemoglobin was added to scavenge Cd not bound to MT. The hemoglobin and excess Cd were separated from the 109Cd-MT by heating. The Cd remaining in the supernatant was a quantitative measure of the MT present. Analysis of Sephadex G-75 and DEAE-ion exchange fractions of liver supernatants from Cd and Zn-treated rats indicated that the assay is specific for MT. Levels of MT in six rat tissues were quantitated in untreated and Cd-treated rats and were consistent with previous studies on tissue levels of MT. Because of its speed and sensitivity, this assay may be useful for measuring MT in large studies where time and expense are limiting factors.
一种新方法经过改进和评估，作为一种简单、灵敏且定量的程序用于测量组织中的金属硫蛋白（MT）。将组织匀浆经热变性的胞质部分与¹⁰⁹Cd一起孵育，并加入牛血红蛋白以清除未与MT结合的Cd。通过加热将血红蛋白和过量的Cd与¹⁰⁹Cd-MT分离。上清液中剩余的Cd是存在的MT的定量指标。对镉和锌处理大鼠肝脏上清液的葡聚糖凝胶G-75和DEAE离子交换部分的分析表明，该测定法对MT具有特异性。对未处理和镉处理大鼠的六种大鼠组织中的MT水平进行了定量，结果与先前关于MT组织水平的研究一致。由于其速度和灵敏度，该测定法可能有助于在时间和费用是限制因素的大型研究中测量MT。
