钙调蛋白在人多形核白细胞中的免疫荧光定位
Immunofluorescence localization of calmodulin in human polymorphonuclear leukocytes.
作者信息
Marone G, Columbo M, Bianco P, Iervoglini A, Condorelli M
出版信息
Immunol Lett. 1983 May;6(5):283-5. doi: 10.1016/0165-2478(83)90019-6.
Human polymorphonuclear leukocytes (PMNs) were purified (approximately equal to 99%) from peripheral blood of normal, adult volunteers. The indirect immunofluorescence technique was used to investigate the presence and the localization of calmodulin in human PMNs. The cellular distribution of calmodulin has been evaluated using an affinity chromatography-purified sheep IgG anti-calmodulin and fluorescein-conjugated rabbit anti-sheep IgG. The anti-calmodulin immunofluorescence pattern suggests that calmodulin is evenly distributed throughout the cytoplasm of human PMNs.
从正常成年志愿者的外周血中纯化出人类多形核白细胞（PMNs）（纯度约为99%）。采用间接免疫荧光技术研究钙调蛋白在人类PMNs中的存在及定位情况。使用亲和层析纯化的羊抗钙调蛋白IgG和异硫氰酸荧光素标记的兔抗羊IgG来评估钙调蛋白的细胞分布。抗钙调蛋白免疫荧光模式表明，钙调蛋白均匀分布于人类PMNs的整个细胞质中。
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