Paull W K, King J C
Histochemistry. 1983;78(3):413-6. doi: 10.1007/BF00496629.
This paper describes the construction and use of a washing/incubation chamber that may be used for peroxidase-antiperoxidase immunocytochemistry of vibratome sections. The advantages of the chamber include: all sections are processed simultaneously with exception of exposure to the primary antiserum; the exposure of the sections to DAB substrate can be precisely controlled; and also the sections are not directly manipulated during the procedure resulting in a much greater integrity of the tissue. Use of the chamber reduces the amount of labor normally required to wash the sections throughout the procedure. Application of this system to the development and standardization of quantitative immunocytochemical techniques is also discussed.
本文描述了一种可用于振动切片机切片的过氧化物酶-抗过氧化物酶免疫细胞化学的洗涤/孵育室的构建和使用。该室的优点包括：除了与一抗接触外，所有切片同时进行处理；切片与二氨基联苯胺（DAB）底物的接触可得到精确控制；并且在操作过程中不直接对切片进行操作，从而使组织的完整性大大提高。使用该室减少了整个操作过程中通常用于洗涤切片所需的工作量。还讨论了该系统在定量免疫细胞化学技术的开发和标准化中的应用。
