Nusbickel F R
J Microsc. 1980 Apr;118(4):447-51. doi: 10.1111/j.1365-2818.1980.tb00294.x.
Many histochemical procedures, in an attempt to be as variable-free as possible, often require such a quantity of incubation solution that there is a resulting waste of expensive chemical substrates, coupling salts, antigens, etc. More economic procedures are justifiably ignored in histochemical studies because of their uncontrollable variables. However, the variables associated with one such economic procedure (the drop method) can now be eliminated by utilizing the closed-chambered incubator described in this paper. Slides containing tissue sections incubated in this apparatus need only 1 ml of incubation medium per slide to achieve a similar reaction product as tissue sections emersed in Coplin jars of medium. This incubator incorporates a water-bath circulator to heat and circulate water below a shelf containing the tissue sections. Within 0.5 h, the entire incubating chamber is saturated with a 310 K water vapour. The water vapour eliminates the primary variable of the drop method incubation, evaporation of the medium. This paper describes the construction of the incubator, its evaluation, the savings obtained from one simple histochemical procedure and its applicability in enzyme and the immunoperoxidase histochemical techniques.
许多组织化学方法为了尽可能减少变量，常常需要大量的孵育溶液，从而导致昂贵的化学底物、偶联盐、抗原等的浪费。由于存在无法控制的变量，在组织化学研究中，更经济的方法往往被理所当然地忽视。然而，现在可以通过使用本文所述的密闭腔室孵育器来消除与一种这样的经济方法（滴加法）相关的变量。在该仪器中孵育的含有组织切片的载玻片，每张只需1毫升孵育培养基，就能获得与浸在Coplin氏瓶培养基中的组织切片相似的反应产物。该孵育器装有一个水浴循环器，用于加热和循环位于装有组织切片的架子下方的水。在0.5小时内，整个孵育腔室被310K的水蒸气饱和。水蒸气消除了滴加法孵育的主要变量，即培养基的蒸发。本文描述了孵育器的构造、评估、从一个简单的组织化学方法中获得的节省以及它在酶和免疫过氧化物酶组织化学技术中的适用性。
