Beuvery E C, van Delft R W, Tiesjema R H, Nagel J
J Biol Stand. 1983 Jul;11(3):195-204. doi: 10.1016/s0092-1157(83)80006-7.
A non-competitive enzyme-linked immunosorbent assay (ELISA) system has been developed for the quantitative, sensitive and specific determination of meningococcal polysaccharides. A prerequisite, however, is the correspondence of the O-acetyl content and the molecular size of the polysaccharides in the samples and the reference preparation. If these characteristics differ, special precautions in the use of antibodies to both the O-acetylated and the non-O-acetylated variants have to be taken, including the testing of the sample in at no less than three dilutions.
已开发出一种非竞争性酶联免疫吸附测定（ELISA）系统，用于定量、灵敏且特异测定脑膜炎球菌多糖。然而，一个前提条件是样品和参比制剂中多糖的O - 乙酰基含量及分子大小需一致。如果这些特性不同，在使用针对O - 乙酰化和非O - 乙酰化变体的抗体时必须采取特殊预防措施，包括对样品进行不少于三种稀释度的检测。
