Brooks R F, Richmond F N
J Cell Sci. 1983 May;61:231-45. doi: 10.1242/jcs.61.1.231.
When Swiss 3T3 cells are treated with colcemid for 60 min (0.5-2 micrograms/ml), although the cytoplasmic network of microtubules is considerably reduced, a significant but variable number of microtubules remain. These residual microtubules appear to converge on a single, usually ciliated, organizing centre. In contrast, on removal of the colcemid, microtubule regrowth takes place from two organizing centres in approximately 50% of the cells. Surprisingly, whether a cell has one or two organizing centres during recovery does not depend on its position in the cell cycle, though recovery takes place earlier in serum-stimulated cells than in serum-starved quiescent ones. It seems likely that prior to colcemid treatment, cells contain two potential organizing centres only one of which is normally active, the inactive one becoming active after exposure to the drug.
当用秋水仙酰胺（0.5 - 2微克/毫升）处理瑞士3T3细胞60分钟时，尽管微管的细胞质网络显著减少，但仍有相当数量的微管残留，数量可观但有所变化。这些残留微管似乎汇聚于一个单一的、通常有纤毛的组织中心。相比之下，去除秋水仙酰胺后，约50%的细胞中微管从两个组织中心开始重新生长。令人惊讶的是，恢复过程中细胞有一个还是两个组织中心并不取决于其在细胞周期中的位置，不过血清刺激的细胞比血清饥饿的静止细胞恢复得更早。在秋水仙酰胺处理之前，细胞似乎含有两个潜在的组织中心，其中只有一个通常处于活跃状态，暴露于药物后，不活跃的那个会变得活跃。
