Watt F M, Sidebottom E, Harris H
J Cell Sci. 1980 Aug;44:123-33. doi: 10.1242/jcs.44.1.123.
Immunofluorescence studies showed that most binucleate Vero cells formed by virus-induced fusion or by inhibition of cytokinesis had a single microtubule-organizing centre (MTOC) when examined during the reassembly of microtubules after chilling, but two or more organizing centres when examined after exposure to colcemid. These findings suggest that although binucleate cells initially contain more MTOC than mononucleate cells, the extra MTOC are normally aggregated, so that the number of MTOC in binucleate cells tends to be reduced very quickly to that in mononucleate cells.
免疫荧光研究表明，通过病毒诱导融合或抑制胞质分裂形成的大多数双核Vero细胞，在冷冻后微管重新组装过程中进行检查时，具有单个微管组织中心（MTOC），但在接触秋水仙酰胺后进行检查时，则有两个或更多组织中心。这些发现表明，尽管双核细胞最初含有的MTOC比单核细胞多，但额外的MTOC通常会聚集，因此双核细胞中MTOC的数量往往会很快减少到单核细胞中的数量。
