Mitchell D M, Callard R E
J Immunol. 1983 Sep;131(3):1229-33.
The fine specificity of anti-influenza antibody produced in vitro by human PBM stimulated with different strains of influenza virus was examined by competition binding in solid phase enzyme immunoassay. Most of the antibody produced in vitro is directed to strain-specific or cross-reactive determinants on the hemagglutinin molecule. The extent of cross-reactivity is dependent on the strain of virus used to stimulate PBM as well as the individual tested and presumably on his previous exposure to influenza viruses. PBM from some individuals produced antibody that bound to the stimulating strain of influenza virus but not to other strains of the same subtype. In other individuals, antibody was produced in vitro that cross-reacted with all viruses in the same subtype (e.g., H3N2; A/X31, A/X47, and A/Texas) but did not bind to other (H2N1 or H1N1) subtypes, and in a few individuals, extensive cross-reaction between subtypes was seen. The presence of antibody to hemagglutinin in these culture supernatants was confirmed by competition binding to highly purified hemagglutinin. This in vitro culture system allows the immunologic memory of individuals to a wide range of stimulating virus strains to be examined simultaneously in terms of specificity of the antibody response by human PBM to influenza virus after natural infection or immunization.
采用固相酶免疫分析中的竞争结合法，检测了不同流感病毒株刺激人外周血单核细胞（PBM）体外产生的抗流感抗体的精细特异性。体外产生的大多数抗体针对血凝素分子上的株特异性或交叉反应性决定簇。交叉反应的程度取决于用于刺激PBM的病毒株以及被检测个体，可能还取决于其既往接触流感病毒的情况。一些个体的PBM产生的抗体与刺激用的流感病毒株结合，但不与同一亚型的其他病毒株结合。在其他个体中，体外产生的抗体与同一亚型的所有病毒（如H3N2；A/X31、A/X47和A/得克萨斯）发生交叉反应，但不与其他（H2N1或H1N1）亚型结合，少数个体中还观察到亚型间的广泛交叉反应。通过与高度纯化的血凝素竞争结合，证实了这些培养上清液中存在抗血凝素抗体。该体外培养系统能够同时检测个体对多种刺激病毒株的免疫记忆，具体表现为人PBM在自然感染或免疫后对流感病毒抗体反应的特异性。
