放射免疫分析法与酶联免疫吸附测定法检测染色质成分抗体的比较

Comparison of radioimmunoassay and ELISA methods for detection of antibodies to chromatin components.

作者信息

Fowler E, Cheng N

出版信息

J Immunol Methods. 1983 Sep 16;62(3):297-303. doi: 10.1016/0022-1759(83)90173-4.

Abstract

A solid phase radioimmunoassay has been compared with an enzyme-linked immunosorbent assay (ELISA) for efficacy in measuring anti-chromatin antibodies. The low backgrounds achieved with the radioimmunoassay method produced a high signal-to-noise ratio and enabled detection of the human test antiserum at a dilution of 1:102,400. By contrast, the ELISA could detect the same antiserum only at a dilution of 1:3200 and above. The radioimmunoassay was consistently more sensitive than the ELISA for detection of anti-chromatin antibodies in a number of human and mouse sera and ascites fluid containing a monoclonal antibody. Factors affecting sensitivity in both assays are discussed.

摘要

已对固相放射免疫测定法和酶联免疫吸附测定法（ELISA）在检测抗染色质抗体方面的功效进行了比较。放射免疫测定法获得的低背景产生了高信噪比，并能够在1:102,400的稀释度下检测人测试抗血清。相比之下，ELISA只能在1:3200及以上的稀释度下检测到相同的抗血清。在检测多种人血清、小鼠血清以及含有单克隆抗体的腹水中的抗染色质抗体时，放射免疫测定法始终比ELISA更灵敏。文中还讨论了两种测定法中影响灵敏度的因素。